DNA酶I 來源于牛胰腺(重組), ≥5000 units/mg protein
Product Name： Deoxyribonuclease I from bovine pancreas(Recombinant)
別名:脫氧核糖核酸酶 I;脫氧核糖核酸 5′-寡核苷酸-水解酶
CAS號(hào):9003-98-9
用途： 用于從蛋白質(zhì)樣品中除去 DNA。 Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I.
貯存： 儲(chǔ)存溫度2-8℃
敏感性： 易潮濕
生化和生理學(xué)機(jī)理： DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. The pH optimum is found to be between 7 and 8. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4：1：1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS)6 and actin7 are known to inhibit the enzyme activity.
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