溶菌酶 來(lái)源于雞蛋白, ≥5,000 units/mg dry weight
Product Name： Lysozyme from chicken egg white
別名:粘肽 N-乙酰胞壁質(zhì)水解酶;胞壁質(zhì)酶
CAS號(hào):12650-88-3
介紹： Lysozyme preferentially hydrolyzes the β-1,4 glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. A slightly more limited activity is exhibited toward chitin oligomers. It has a molecular weight of 14,388 daltons. Optimum pH is 9.2. Lysozyme is inhibited by surface active agents such as dodecyl sulfate, alcohol and fatty acids. Imidazole and indole derivatives form inhibitory charged transfer complexes.
用途： 酶分解細(xì)菌的細(xì)胞壁；用于制備原生質(zhì)球。
貯存： 儲(chǔ)存溫度2-8℃
生化和生理學(xué)機(jī)理： 溶菌酶水解肽聚糖中 N-乙酰胞壁酸和 N-乙?；?D-葡糖胺殘基間的 β(1→4) 鍵以及殼糊精中 N-乙酰基-D-葡糖胺殘基間的 β(1→4)鍵。革蘭氏陽(yáng)性細(xì)胞對(duì)該水解作用非常敏感，因?yàn)樗鼈兊募?xì)胞壁含有較高比例的肽聚糖。由于存在外膜以及比例較低的肽聚糖，革蘭氏陰性細(xì)菌對(duì)該水解作用不太敏感。然而，細(xì)菌外膜中如果存在與金屬離子螯合的 EDTA，那么這些細(xì)胞也可能被水解。 該酶在較寬的 pH 范圍（6.0 到 9.0）內(nèi)具有活性。與 pH 為 9.2 時(shí)相比，pH 為 6.2 時(shí)可在更廣的離子強(qiáng)度（0.02 到 0.100M）范圍內(nèi)觀察到最大活性。
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